Course

Course Summary
Credit Type:
Course
ACE ID:
DLSI-0039
Organization's ID:
DLSI-0039
Organization:
Distance Learning Systems Indiana, Inc
Location:
Online
Length:
8 weeks (144 hours).
Dates Offered:
Credit Recommendation & Competencies
Level Credits (SH) Subject
Lower-Division Baccalaureate 1 Microbiology Laboratory
Description

Objective:

This stand-alone lab course can be used alongside a Microbiology lecture. This lab course initiates the application of Microbiology by having students complete virtual laboratory simulations, directed by step-by-step instructions that mirror real life experiments. Course learning objectives and lab methodologies are explored through the use of in-lab evaluation questions. Microbiology is a lab course intended for students interested in learning about the microbial world and how the microbial world influences human actions and interactions. Specific topics covered include Laboratory Safety, Virtual Microscopy, Diversity of Microorganisms, Utilization and Application of the Aseptic Technique, Gram Staining, Acid-fast Staining, Bacterial Smear Sample Preparation, Bacterial Isolation Methods, Influences of Environmental Factors on Microbial Growth, Ubiquity of Microorganisms, Antiseptics & Disinfectants, and Kirby-Bauer Testing. * This lab course is not intended for Allied Health majors.

Learning Outcomes:

  • Correctly utilize laboratory equipment (such as a virtual brightfield microscope) and stains (such as Crystal Violet and Gram’s Iodine) as indicated.
  • Communicate results of scientific investigations, analyze data, and formulate conclusions.
  • Compare, contrast, and categorize the diversity of microorganisms.
  • Administer the aseptic technique virtually and recognize why it is necessary in a lab setting.
  • Distinguish between various bacterial isolation methods and identify how they are used.
  • Recognize and complete microbial laboratory techniques and evaluate when to use certain techniques.
  • Identify, distinguish, and apply how environmental factors including temperature, pH, availability of oxygen, and osmotic pressure affect microbial growth.
  • Identify and demonstrate virtually the ubiquity of microorganisms.
  • Recognize and apply how antiseptics, disinfectants, and antibiotics are used to control microbial growth.
  • Apply correct safety standards.

General Topics:

  • Virtual Labs Tutorial Lab Safety - Personal Safety Lab Safety - Handwashing Procedure Microscopy - Operation of Brightfield Microscope
  • Microscopy - Diversity of Microorganisms Microscopy - Pond Water Wet Mount Aseptic Technique - Broth Culture to Sterile Agar Plate Aseptic Technique - Broth Culture to Sterile Broth Aseptic Technique - Slant Culture to Sterile Agar Slant Staining - Gram Staining Staining - Acid-Fast Staining Staining - Preparing a Smear Sample from a Bacterial Sample Isolation Methods - Quantitative Dilution of Bacteria Isolation Methods - Quantification by Colony Counting Isolation Methods - Quadrant Streak Plate Method Microbial Growth - Effects of pH Microbial Growth - Effects of Temperature Microbial Growth - Effects of Osmotic Pressure Microbial Growth - Oxygen Requirements and Anaerobic Jar Microbial Growth - Oxygen Requirements and Fluid Thioglycolate Medium Tubes Ubiquity of Microorganisms - Sampling Surfaces for Bacteria Control of Microbial Growth - Effect of Antiseptics and Disinfectants Control of Microbial Growth - Antimicrobial Sensitivity (Kirby-Bauer Method)
Instruction & Assessment

Instructional Strategies:

  • Audio Visual Materials
  • Discussion
  • Laboratory
  • Practical Exercises

Methods of Assessment:

  • Other
  • Quizzes
  • Virtual Lab Submissions

Minimum Passing Score:

70%
Supplemental Materials